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plasmid based mixes  (Addgene inc)


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    Addgene inc plasmid based mixes
    Plasmid Based Mixes, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+based+mixes/pm41896213-183-11-22?v=Addgene+inc
    Average 93 stars, based on 56 article reviews
    plasmid based mixes - by Bioz Stars, 2026-07
    93/100 stars

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    Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with <t>Yep24</t> plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .
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    Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with Yep24 plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .

    Journal: Nucleic acids research

    Article Title: Splicing factor Prp18p promotes genome-wide fidelity of consensus 3'-splice sites.

    doi: 10.1093/nar/gkad968

    Figure Lengend Snippet: Figure 5. Slu7p is necessary to recruit Prp18p but not sufficient to fulfill its 3 ′ SS fidelity functions. ( A ) Spot dilution growth assay for wild-type and prp18 upf1 strain complemented with Yep24 plasmids expressing PRP18 or o v ere xpressing SLU7 or empty Yep24 (vector). ( B ) RT-PCR analysis of NYV1 alternative 3 ′ SS splicing in WT, upf1 , prp18 and prp18 upf1 strains transformed with a vector (–) or a plasmid overexpressing SLU7 (+). ( C ) R T-PCR analy sis of NYV1 and MUD1 alternativ e splicing in v arious slu7 mutants. All strains are in a upf1 back ground. ( D ) Quantification of the ratio of usage of the major alternative 3 ′ SS of NYV1 and MUD1 relative to the main 3 ′ SS in the various slu7 mutants. Legends as in Figure 4 .

    Article Snippet: Construction of the PRP18 / pUG35 and PRP18 / YEp24 base plasmids was accomplished using Gibson assembly ( 23 ) ( New England Biolabs #E2611 ) .

    Techniques: Growth Assay, Expressing, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Transformation Assay